The Value Of A Brand For A Product Or Service - 1508 Words

High end luxury, extravagance is the core of Elie Saab’s business. The brand has become a symbol of indulgence for the finer luxuries. At the essence, it is one of the few haute couture brands which is an added sense of elegance and importance. The brand focuses on those consumers who look toward the augmented value of the product and not the product itself. The representation of ES as a brand for the affluent and glamorous is the intangible benefit offered through its products. The decision to pay money for a product or service is often based on more than just the product or service itself. Consumers care deeply about the overall experience of the buying process: They respond to the marketing message, the advertising, the sales approach, the website, the interaction with company personnel, and more. When all these elements come together to form a seamless experience, the customer is left with a feeling of satisfaction that ultimately builds loyalty† (Joseph, 2010). The actual product offered by Saab in haute couture is one created through personalized effort and a guaranteed lifelong experience. ES has four product lines – its essence and the representation of its brand name through Haute Couture. A Ready-to-Wear collection (RTW) that is updated every season and that has become an integral part of the ES experience. The third is Accessories which include shoes, handbags, jewelry, fragrances etc. and the fourth line is Wedding Dresses which are created as Haute Couture orShow MoreRelatedMarketing Is The Flow Of Product Value, Service And Brand Essay2478 Words   |  10 PagesDefinition of marketing: Marketing is the flow of product value, services to the public, the main purpose is to promote the interests of product sales, service and brand. Marketing as a system to provide information to the public dissemination and support Definition of marketing research: Market research is to connect with consumers, public and customers by information. To identify and define the problems and opportunities of market information. Generate and evaluate marketing activities. MarketRead MoreMarketing Means Communicating The Value Of Product, Service And Brand Essay3910 Words   |  16 PagesMarketing means communicating the value of product, service or brand to customer, for the purpose of promoting or selling that product, service or brand. Mostly company do marketing so that people can come to new about their company and it help them to increase the rate of customers and profit for the company. Product:- While doing the market, company must show the description about their product what product they provide to the customers and what type of product they provide such as vegetarian, non-Read MoreDiscuss the Concept of Perceived Value and Its Importance to Consumer Behaviour and Marketing. Discuss the Theory and Then Give Practical Examples of How Customers Perceive Various Brands and How This Impacts on Their Behaviour.1161 Words   |  5 PagesThe concept of perceived value of a product is the difference between the potential customers assessment of all the benefits and all the costs of an offering compared to the perceived alternatives. Value is the function of consumer’s evaluation thus it is subjective where the cost and benefit must be positive values. We can express as the following equation: Value = Benefits / Cost The consumers perceived value of a good or service affects the price that he or she is willing to pay for it. ForRead MoreCustomer Value : Customer Perceived Value1521 Words   |  7 PagesCustomer Perceived Value According to Chen and Lin (2015), perceived value is the worth, which a service or a product has within the mind of the consumer. In reality, the consumers perceived value of the service or product affects the price, which she or he is willing to pay for it. De Oliveira et al (2015) state that customer perceived value is the anticipated benefit from the consumer’s perspective of the service or product. Notably, customer perceived value stems from the psychological, tangibleRead MoreMarketing Management : Starbucks Vision And Mission1018 Words   |  5 Pagesbusinesses create value. It involves redefining the way organizations engage individuals in value creation, especially employees and internal stakeholders, but also customers, suppliers, and related external stakeholders and communities. It is about organizations unleashing the creative vitality of people by inviting and enabling them to interact with them differently† (Experience Co-Creation Partnership, 2015, p. 1 ).The goal of co-creation is about engaging people and creating value through their experiencesRead MoreChapter 9 Product : The Online Offer1332 Words   |  6 PagesWeek 2 Chapter 9 Product: The Online Offer In today’s economy, a product is anything that can be purchase physically or virtually. According to the book, a product is any tangible good, service, idea, people, and place. They are products because in one way or another they could be marketed on the Internet. A product is not just born out of nowhere; a product is created through research in order to determine what is important to the consumer. The marketing mix consists of product, place, distributionRead MoreMarketing Concepts Based On An Article Essay1488 Words   |  6 Pageswill discuss three out of five marketing concepts that implies between price, product and brand. The most important concept based on the article is prices which will represent marketers’ value in the product or services and maintain demand and supply relationship. Introduction This business report is focusing on marketing concepts as a fundamental and motivation of a firm which had been done when they offer their products to the market as well as what needs to improve to make it better in the competitionRead MoreWalmart : A Brand As A Strong Brand859 Words   |  4 Pagesconsumers while earning profits though volume. (Frank, 2006) The company sells varieties of products from clothing wear to food products and more. Today, Walmart has over five thousand stores located in the United States and over six thousand stores across the nation. (Statista, n.d.) Walmart’s strategies had helped the company became a strong brand. Additionally, Walmart’s offering various products/services is the company’s strategy to expand its growth. Walmart may have experienced some negativeRead MoreThe Role Of Product And Brand Strategy Essay1129 Words   |  5 PagesIntroduction: The role of the Product Brand becomes more precious when the economic and industry in stability continue to impact Business to Business buying and selling. Kotler (2001) Product is anything that can be offered to a market for attention, acquisition, use or consumption that might satisfy a want or need. As research has explained the customers’ needs and wants, as well as their tastes preferences, such that it becomes easy for the companies to understand their customers. For achievingRead MoreConvenience Store1483 Words   |  6 Pages7-Eleven and OK convenience stores to analyze. In this part, I am going to identify the distinctive characteristics and values of 7-Eleven and OK with the theory of brand triangle, to see what make them a brand. And then I will analyze the scale and goals of 7-Eleven and OK convenience stores with the theory of SWOT and 4P, to see how they manage theirs business. †¢ Corporate Brand Analysis | |Image |Identity |Positioning

Southwest s The Dominant Airline Beloved By Shareholders,...

For years I traveled around the country for business and pleasure, Southwest quickly became my favorite airline. At that time with a young son and parent in New Hampshire, they had a non-stop flight from Kansas City to Manchester. I recall on one flight the flight attendant while descending over the PA sang the following: â€Å"I love you, you love us we’re much faster than a bus.† This song embodies the spirit of Southwest and why I mention this story from over a decade ago. I want to ascertain what make Southwest the dominant airline beloved by shareholders, customers, and employees? It is one keyword, communication. Southwest stresses it is important to deliver a first-class customer experience that will lead to return clients is a strength. To adhere to this tenant, they established a Listening Center with staff from customer relations, communications, and marketing to expedite the flow of information and resolve issues quickly. Communication is a vital comp onent and strength at Southwest starting with the top executives including former Chief Executive Officer and Co-Founder Herb Kelleher. Southwest dares to differ from other carriers because their philosophy revolves around people both internal and external. They use a two-way symmetrical model of communication to forge satisfactory connections between organizations and their stakeholders (Grunig, Grunig, Dozier, 2006). The principle revolves around understanding the shareholder and making sure both theShow MoreRelatedThe Role Of Communication And Culture At Southwest3857 Words   |  16 PagesIntroduction â€Å"Your employees come first, and if you treat your employee’s right, guess what? Your customers come back, and that makes your shareholders happy. Start with employees and the rest follows from that† (Byrne, 2012). This quote from Herb Kelleher, co-founder of Southwest Airlines, embodies the leadership at the airline. Southwest is the leading low-cost airline based in Dallas, Texas and founded in 1967 by Herb Kelleher and Rollin King. They dare to differ from other carriers becauseRead MoreExploring Corporate Strategy - Case164366 Words   |  658 Pagesmission. Bayer MS – corporate social responsibility in the international development of a German company. Eurotunnel – clash of cultures threatens to derail Anglo–French rail link. Ryanair – competitive challenge and strategic choice in the budget airline industry. IKEA – quality and low prices at the Swedish furniture giant News Corporation – corporate logic and corpo rate management in a worldwide media business. CRH – impressive international growth of an Irish company driven from a ‘lean’ corporateRead MoreInnovators Dna84615 Words   |  339 Pages 6:52 PM Page ii 100092 00 i-vi r1 rr.qxp 5/13/11 6:52 PM Page iii THE INNOVATOR’S DNA MASTERING THE FIVE SKILLS OF DISRUPTIVE INNOVATORS Jeff Dyer Hal Gregersen Clayton M. Christensen H A R VA R D B U S I N E S S R E V I E W P R E S S BOSTON, MASSACHUSETTS 100092 00 i-vi r1 rr.qxp 5/13/11 6:52 PM Page iv Copyright 2011 Jeff Dyer, Hal Gregersen, and Clayton M. Christensen All rights reserved Printed in the United States of America 10 9 8 7 6 5 4Read MoreBrand Building Blocks96400 Words   |  386 PagesBRAND BUILDING BLOCKS Building Strong Brands: Why Is It Hard? It is not easy to build brands in today s environment. The brand builder who attempts to develop a strong brand is like a golfer playing on a course with heavy roughs, deep sand traps, sharp doglegs, and vast water barriers. It is difficult to score well in such conditions. Substantial pressures and barriers, both internal and external, can inhibit the brand builder. To be able to develop effective brand strategies, it is useful toRead MoreMarketing Management 14th Edition Test Bank Kotler Test Bank173911 Words   |  696 Pagesand services. C) It helps to build a loyal customer base but has no impact on a firms intangible assets. D) It is more important for bigger organizations than smaller ones. E) It is seldom used by nonprofit organizations. Answer: B Page Ref: 4 Objective: 1 Difficulty: Easy 2) ________ is the art and science of choosing target markets and getting, keeping, and growing customers through creating, delivering, and communicating superior customer value. A) Marketing management B) Knowledge

Services Marketing Integrating Customer Focus

Question: Discuss about theServices Marketing for Integrating Customer Focus. Answer: Introduction: Taking into consideration the marketing mix strategy of Qantas Airways Limited and flag carrier airline of Australia for carrying passengers to and fro and flying under the Qantas brand. Being the largest airline company in Australia, it carries considerable 30 million number of annual passenger in and out (Bienstock, Mentzer Kahn, 2015). Qantas marketplace consists of international and domestic passengers characterized by a cut throat competition. Since the company has a monopoly over the market almost 60 regional locations are serviced by Qantas Link. Despite of its competitors like Virgin Australia, Regional Express Airlines and SkyWest its popularity is unquestionable (FISK, 2013). 3P of Marketing Promotion In the case of marketing mix, promotion provides the trend in aspects of marketing communication. Therefore, absolute information is required in order to generate positive response from customer. In terms of advertising agents like TV, radio, brochures, magazines etc. they reflect the purpose of branding and also using the customized message to hit them at personal level. Another activity like sales promotion is usually consumed during low seasonal changes (Lovelock Patterson, 2015). Moreover social networking and email marketing are getting closer to customer these day, they imprint higher efficiency. PR activities, engagement with sports, and event art and other related programs, and various other involvement are used for charitable purpose. Qantas airlines needs to keep the image of the country, its scenic beauty, tourist attraction, culture heritage that would attract number of tourists. As a public representative like travel agents, media people they publicize the business (Wi rtz Lovelock, 2016). Even the tour operators, frontline staffs contribute lot to the promotion. As such, Qantas brand name holds as a powerful marketing tool and helps distinguishing Qantas from their competitors thereby attracting international customers to have a well-known brand name. It also participates in short term inducement for increasing sales promotion particularly in period of dejected demands (Shani Chalasani, 2013). The generation of new channels of media along with the internet has led to diminish the large scale advertisement and thus Qantas has started to focus on other promotional strategies. People Most customers come in contact with Qantas employees that give a big impact over how it is taken into perception. Qantas being a service industry consists of two different customers: external customer that are the passengers and internal customer as employees. Here the cabin crew plays a major role as they deliver the services and thus are the walking billboards of every organization (Wilson et al., 2012). It provides uniform to improve the professional appearance thereby implementing effective process and ensuring efficient customer friendly. Because of competent, reliable and caring attitude they make an impression with everyone they come in contact and so these staff members has profound effect over customer satisfaction. Even the reputation of the brand must be well suited for the role. It also formulates the policies for online interaction and ground especially through ticketing, check-in and baggage handling and making sure of everyone presence. For making happy customer and ex cellent advocates it provides responsiveness, is initiative, can solve problem and have good will (Lovelock Patterson, 2015). Thus the quality of Qantas services mostly depends upon the human resources capital. Having around 33000 staffs the company provides appropriate personal attributes and relevant training by spending around $275 million a year on staff training (Kumar et al., 2013). Qantas also demonstrates its adherence to consumer willingness over its influence through its service choice and specialist for those accompanied with varied culture. Process Qantas implements effective processes and ensures delivering the product or services in a timely and fashionable manner. Usually an airline is judges based upon its efficient and customer friendly possessions (Lovelock Patterson, 2015). In the same way for the company has invested an entire experience that starts from the moment of discovering and stays till purchase and beyond. Important sources for consideration lying here are reservation, baggage handling, facilities at Airport, information regarding flight, entertainment in flight, meal services, delivering quality service, etc. For example Q Bag tags have allowed passengers to check in baggage without attaching the bag tags (Wirtz Lovelock, 2016). This process is convenient and stores your flight details there by letting your bags to be easily traceable. Few more efficient processes are booking flights online (accompanied by searching for available flights and passenger details and payment), online check in (selecting passenge r and flights, change seats any warning and printing boarding pass), and mobile check in (involves visiting the website and selecting domestic check in, retrieving the booking. References Bienstock, C.C., Mentzer, J.T. and Kahn, K.B., 2015. How are Service Firms Measuring and Managing Service Quality/Customer Satisfaction?. In Proceedings of the 1996 Academy of Marketing Science (AMS) Annual Conference(pp. 161-161). Springer International Publishing. FISK, R.P., 2013. Lmpression management 26 in services marketing: A Dramaturgical Perspective.Impression management in the organization, p.427. Kumar, V., Chattaraman, V., Neghina, C., Skiera, B., Aksoy, L., Buoye, A. and Henseler, J., 2013. Data-driven services marketing in a connected world. Journal of Service Management,24(3), pp.330-352. Lovelock, C. and Patterson, P., 2015.Services marketing. Pearson Australia. Shani, D. and Chalasani, S., 2013. Exploiting niches using relationship marketing.Journal of Services Marketing. Wilson, A., Zeithaml, V.A., Bitner, M.J. and Gremler, D.D., 2012.Services marketing: Integrating customer focus across the firm. McGraw Hill. Wirtz, J. and Lovelock, C., 2016. Services marketing: people, technology, strategy.

Corporate Governance in Banking Sector free essay sample

Therefore, they require proper accountability of the financial performance of their business. Corporate governance examines the decision process in corporations. It can also be defined as a system and process that ensure accountability, probity and openness in the operation of a corporation. Corporate governance entails the compliance of its various codes of conduct and Government Regulations. As such, companies are required to publish their business activities in their annual reports. However, some companies in UK such as Polly Peck, Bank of Credit and Commercial Bank (BCCI) and Maxwell group, have failed to meet these required regulations. These firms have failed because the directors were acting against shareholders’ interests. Most famous corporate scandals have been companies like Enron, WorldCom and Barings Bank. There was poor governance in these companies, which has been the main reason for their failure. In 1980’s, corporate governance has been a great issue of concern due to big corporate scandals resulting from lack of accountability of board members and weak accounting standards. We will write a custom essay sample on Corporate Governance in Banking Sector or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Enron, the seventh largest American company, known as the giant energy company, has also collapsed due to lack of control and poor governance. In this case, the members of the directors were falsifying the company’s accounts to show a good picture of the position of the firm. Enron ended in bankruptcy due to inadequate internal control, unethical behavior by directors and lack of independence by qualified members. According to Financial Sector Assessment (2003), Mauritius is among the groups of developing countries and its domestic bank assets indicate nearly 100% of its Gross Domestic Product (GDP). The Mauritius banking sector represents two-third of its domestic financial system. And, over the past five years it has increased at an average of 13% per year. The financial sector in Mauritius is normally good and lucrative. There are 19 banks in Mauritius with 173 branches over the country. Apart from domestic banks, in Mauritius there are also big and famous banks such as HSBC, Barclays, Deutsche, Bank of Baroda, State Bank of India, South East Asian Bank, and others. Appendix (i) provides a list of banks operating in Mauritius. Financial Services Authority (FSA), Banks of Mauritius and Code of Governance regulate banks in Mauritius. Mauritius Institute of Directors (MIOD 2008) state that due to corporate scandals in the country a code of recommended best practices were in review so that there would be responsible corporate behavior. Therefore, in 2005, the national code of corporate governance was in use in Mauritius. Everyone in the organization should participate in achieving effective corporate governance. It ranges from shareholders to employees, as they are accountable in reaching the aim and objectives of their business. Sir Adrian Cadbury (World Bank 2000) states, â€Å" the aim is to support as much as possible the interests of individuals, corporations and society†

LDH Purification lab Report Essay Example

LDH Purification lab Report Paper OLD was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometers determination of NADIA at 340 NM. From Pierce BCC assay of crude homogenate, initial protein concentration was shown to be 100 MGM/ml. The final protein concentration of the pooled affinity sample was shown to be 0. 2 MGM/ml. It was found that the total specific activity of OLD was 58. 5 mol/min/MGM, and yield of 0. 6%. Even though we were successful in purifying OLD enzyme, further steps can be taken to increase the yield. Materials and Methods Cell Lysine and Extraction of OLD: Approximately 40 g of minced chicken breast eat (40. 327 g) is blended with ml cold extraction buffer in four 30-seconds bursts for homogenate of the muscle tissue. The extraction buffer contained mm Tries-HCI (pH-7. 4), mm 2-Merchantable, mm Phenylmethylsulfonylflouride (AMPS), 1 mm Ethylene Dianne attracted acid (EDIT). The homogeneities procedure was carried out in the cold room to prevent the denomination of proteins. The homogenate was centrifuged at 15,000 RPM for 20 minutes at 40 C. The supernatant was filtered through two layers of cheesecloth to remove lipids from the supernatant. The total volume was noted and three 0. Ml aliquots (crude extract) were stored at -200 C. Ammonium sulfate precipitation: 60% ammonium sulfate concentration was used to precipitate proteins. 0. 39 g of ammonium sulfate per ml of the supernatant was added gradually to the supernatant for 15-20 min with continuous gentle stirring at 40 C. The mixture was centrifuged for 20 minutes at 1 5,000 RPM at 40 C. The supernatant was discarded and the pellet was stored at -200 c. We will write a custom essay sample on LDH Purification lab Report specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on LDH Purification lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on LDH Purification lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Dialysis: Ammonium precipitation leads to high concentration of salts in protein mixture that can interfere with further purification steps. In order to remove excess salts, dialysis was performed. The pellet was suspended in Tries-AMPS buffer (10 rim Tries-HCI, pH 8. 6, 0. 5 mm 2-Merchantable, and mm ratio of EDIT) and mixed very gently until it dissolved at 40 C. Volume of ml protein mixture was added in the dialysis tubing and incubated twice overnight with two IL buffer changes (Same buffer as extraction buffer that was used for cell lysine). After two incubation, protein mixture was responded gently and centrifuged for 10 minutes at 15,RPM at ICC. Pellet was discarded, total volume of supernatant was noted and three 0. 1 ml aliquots were collected. Affinity Chromatography: Isobaric Blue column was used to separate OLD from the other proteins. Ml fractions were collected in thirteen test tubes. Column was first rinsed with Tries-AMPS buffer followed by addition of protein mixture. Then, ml AND Buffer (mm Tries-HCI pH-8. 6, 0. Mm 2-Merchantable, mm Lithium acetate and 1 mm AND+) was added followed by NADIA (mm Its- HCI PH 8. 6, mm NADIA and 0. Mm 2-Merchantable). Between each steps, column was washed with ml Tries-AMPS Buffer. Each fraction was subjected to absorbency reading of Mann. For absorbency above 1. NM, 1:10 dilutions were carried out. Activity Assay: We used OLD Enzyme assay to measure the amount of OLD activity in our protein mixture. OLD catalysts the conversion of lactate to private and AND+ to NADIA. The NADI A can be determined spectrophotometers at 340 NM. The OLD assay was performed in the crude homogenate, desalted fraction and six peak fractions from the Isobaric blue column. A cocktail solution was prepared by mixing lactate stock solution (120 rim lithium lactate, 10 mm Tries-HCI; pH 8. 6), AND+ stock solution (12 mm AND+, 10 mm Tries HCI; pH 8. 6) and bicarbonate stock solution (18 mm Enhance, 0. 5 M Nasal) in the ratio of in cavetti. 0 micrometers of the sample is then added and the assay absorption is measured at Mann. If absorbency was above 1. 5, samples were diluted. Protein Assay: The Pierce BCC Protein Assay (Thermo Scientific) is a detergent- compatible formulation based on bioscience acid (BCC) for the colorimetric detection and quantization of total protein concentration. A series of standard solution of Bovine Serum Albumin (BAS) ranging from 0-2000 pig/ml was prepared from a stock solution of 2 MGM/ml BAS. Lull of diluted crude (1:500, 1 :250), desalted (1:100, 1:50), and 6 peak fractions from isobaric blue column (1:10, 1:5) ere loaded in microscope along with lull of BCC working reagent. Microscope was incubated for mini at ICC and then the absorbency was measured at Mann. Results/Discussion The purpose of this experiment was to extract and purify OLD enzyme from chicken muscle tissue using a variety of techniques including homogeneities, ammonium sulfate precipitation, dialysis, and affinity chromatography. Activity and Protein assay were used to track the overall amount of OLD present in the samples. Crude Extraction: Chicken muscle tissue was homogeneity in a blender with cold extraction buffer in order to else cells, releasing OLD into slurry of tissue monuments. Centrifugation separated membranes, nuclei, and other large cellular components to a pellet leaving a supernatant of crude product. Controlling temperature was a major consideration after homogeneities since not only did this step releases proteins like OLD from the cell, but it also releases proteases that can now interact to degrade the OLD. Keeping samples on ice, pre-cooling the buffer, and avoiding excess kinetic energy through conservative blending were methods used to minimize activity of these proteases. After filtration through cheesecloth, our final volume of crude homogenate sample ml, much more volume than expected. Addition of more than ml of buffer volume could have increased the volume. Other possible explanation is that more solid components such as fats were present in the sample and hence, more than 20 minutes of centrifugation was required. Desalted Sample: 60% ammonium sulfate is added to the crude extract that precipitates OLD proteins. The resulting 40% pellet theoretically contains most of the original OLD, which is re-suspended in very less volume (ml) to create a more concentrated sample. This process leads to high concentration of salts in rotten mixture that can interfere with subsequent purification steps. Ml protein mixture underwent dialysis procedure that removes excess salts and our final volume after dialysis was ml. One possible explanation for increase in our volume could be that extraction buffer got mixed with protein mixture either due to tubing leaking or tubing clips not being properly tightened. Affinity Chromatography: Isobaric Blue column is an affinity column, which is specific to dehydrogenate type proteins, due to a compound structurally similar to NADIA being attached covalently attached to the column. 13 fractions were elected and absorbency was measured at Mann to check presence of OLD protein in the fractions. 1:10 dilution was performed if absorbency reading was above 1. NM since it spectrographically indicates saturation and less than 1% light reaching the detector. During the addition of protein mixture (fraction# 4), high absorbency reading of NM was obtained (Fig. 1). This could be due to lot of non-dehydrogenate-type proteins present in our sample that got eluted first during affinity chromatography. Second peak was seen after AND+ was added since AND solution results in the removal of the loosely bound protein. Third peak was seen after NADIA was added since NADIA solution results in release of maximum OLD proteins (Fig l) Enzyme Activity Assay: The OLD activity was measured spectrophotometers by measuring the absorbency of NADIA at 340 NM. Three peak fractions were selected for this assay based on their absorbency values obtained after adding AND+ (fraction# 6, 7, 8) and other three after adding NADIA in the affinity chromatography step (fraction# 10, 1 1 , 12). A huge activity of 141 mol/min/ml was seen at fraction# 7(PUFF ) which indicated that we had lot of proteins present in our sample. Second peak activity was seen t fraction indicating that more OLD proteins is present in this fraction than in fraction# 11 (PUFF) (fig. 1). Based on this information, we selected fraction #10 as for our protein assay. Desalted showed highest activity among all the samples (Tablet ) possible due to errors occurring during dialysis explained previously. Figure 1. Absorbency readings of eluted obtained from affinity chromatography with OLD activity for 6 peak fractions. The desalted fraction was loaded to the Isobaric blue column and proteins were eluted with Tries-AMPS, AND+ and NADIA wash subsequently. The absorbency at 280 NM of eluted were measured after ACH collected fractions. The OLD activity was calculated from the absorbency values obtained at Mann. Protein Assay: We used BCC Pierce Assay to determine protein concentrations in our protein mixture. BAS standard curve was created for series of dilutions ranging from 0-2000 pig/ml and linear graph equation was used to calculate protein concentrations for the samples (Table 1). Based on Table 1, with each subsequent purification step, protein concentration decreases as sample become more concentrated with only OLD protein. Specific activity should increase and total activity should decrease with very purification step as samples get less and less diluted.

The Mystery of Life and Death Essays

The Mystery of Life and Death Essays The Mystery of Life and Death Essay The Mystery of Life and Death Essay The Mystery of Life and Death In the 1995 movie Breather, William Wallace presents lifes uncertainties and how we should live each day when he affirms, Every man dies. Not every man really lives. Sharon Oldies poem, Summer Solstice, New York City, is an ideal representation of this quotation and the questions that we have about how to live our day-to-day lives. This brief poem is about a man who is standing on a rooftop contemplating suicide and the New York City policemen who are attempting to save his life. At first, a brief summary is all that the reader sees in the story, but upon peer analysis, the fragility of life shines through. With each detail, or lack thereof, Olds reveals her views on the uncertainties of this life. The Summer Solstice is known as the longest day of the year, and in placing the setting on the Summer Solstice, Olds presents the reader with a lengthy period of time. In the first line, when Olds says that he could not stand it (1) anymore, it presents the concept of a long day, or in the case of the man, a long life and a long time coming. We do not know what has been happening in the mans life, but whatever it is has led him to desire to commit suicide. The man on the rooftops identity remains a complete mystery throughout the entirety of the poem. There is no physical description of the man; the descriptions in the story are of his actions. The lack of description of the man allows readers to apply the mans actions and the story to themselves, rather than merely reading a poem and remaining on the outside. We barely know anything of the mans story except that by the end of the longest day of the year he could not stand it (1). The question remains: What could the man not stand anymore? Some of us may have felt his pain before and are able to fully relate to feeling suicidal, while others understand the concept of being increasingly upset with someone or something. In her word choice, Olds invites the reader to identify with the man and his struggle, whatever our identification with the matter may be. As the man is standing on the roof, he is poised between life and his immediate death. When the man reaches the edge of the roof, without hesitation, he putts] one leg over the complex green tin cornice (4). Hesitation begins to rush over him once he puts his leg over the edge. As the mans leg [hangs] over the lip of the next oral (18), the readers are given strong imagery of how quickly ones life can change. One half of his body is pointed toward life, while the other half of his body is on the side of death; any quick movement in each direction could determine the mans life. This description compares to our lives directly; any action that we take could determine our lives forever. This mystery of death is what attracts all of the people below; they are prepared for the worst. The people [gather] in the street, silent (19) as the cops came in their suits blue-gray (7); they watch as the hairy net with its implacable grid was unfolded near the curb and spread out and stretched as the sheet is prepared to receive at birth (20-22). The scene that is described here is one of waiting on the possibility of death. The policemen are working to prevent what could happen, while the bystanders are awaiting a finale, watching the man balance they are merely interested in the aspect of danger and the fear of someone dangling at such a high altitude with no obvious desire to walk away from the edge. The people standing down below are attempting to place themselves into the mind of the an up on the rooftop understand why he is standing on the edge of the building and why he has not yet Jumped. We are left guessing as to what brings the man to spare his life. Was he actually suicidal? Did the policemen convince him not to take his own life? We are never told the reasons for the mans not Jumping from the building; we only know that everything stopped as his body Jerked and he stepped down from the parapet and went toward them (27-29). We are presented with so few details so that we are able to compare the actions of the man to our own previous experiences or actions. The Nans actions were Just as possible as those which he did not take. The outcome could have been different, and the purpose of the story would remain the same. Life is uncertain, and the line between life and death is extremely thin. As the Summer Solstice marked the official beginning of summer, ancient civilizations used this opportunity to celebrate by throwing festivals and enjoying the day together (Dunlap Patch). At these celebrations, participants would have a bonfire to celebrate the sun and its rays and power (Dunlap Patch). These celebrations connect directly with the ending of the poem, when the policemen have adhered around the man and continue to smoke cigarettes that burned like the tiny campfires we lit at night back at the beginning of the world (38-40). Though the ending of the poem can be interpreted in various ways, the relationship between Summer Solstice, New York City and the holiday for which the poem is named is clear to the reader after further analysis. The comparison between life and death has been depicted in various ways throughout literature and all areas of the arts because audiences are drawn to the mysteries behind the characters. We long for the unknown, as it opens a door to our imagination and creativity. We already have an ending to the story in our heads before the characters can present us with a resolution. By using omission of details, Olds allows us to interpret the poem individually to determine our own views of the world and of our lives. Breather. Dir. Mel Gibson. Peer. Mel Gibson. Paramount Pictures, 1995. Film. Cassia, Ron. Summer Solstice: A Brief History of a Midsummers Traditions. Dunlap Patch. N. P. , 21 Jan. 2011. Web. 24 Gag. 2013.. Olds, Sharon. Summer Solstice, New York City. Making Literature Matter. 5th deed. Deed. John Schlitz and John Clifford. Boston: Bedford, 2012. 21-22.

Amercian History Essay Example | Topics and Well Written Essays - 750 words - 1

Amercian History - Essay Example Although the colonies were self-governed from thirty years earlier, the straining relationship with the British crown became more acute with the passage of each new tax law. The series of tax laws, including the Sugar Act, the Stamp Act and the Currency Act aggravated the grievances of an already over-taxed colonial population. Almost all trade-related shipments to and from the Eastern American coast was deemed taxable. While some of the taxes were reasonable, others were plainly unjust, given that the colonists were not granted representation in the affairs of the Crown. The British Crown reasoned that such taxations were necessary to keep up its expansive and expensive naval operations. But its use of authoritarian methods in enforcing tax laws proved to be the decisive spur for American Revolution. For example, when some of the governing officers in the colonies expressed their solidarity with fellow colonists, the British Crown took a hasty decision to dismiss and replace all dis loyal officers and took more stringent measures to enforce taxation laws. This move further alienated the colonists and primed their thoughts for independence. Studying the American declaration of independence in retrospect, we see that the British rule, by its obstinate, inconsiderate and high-handed approach to dealing with colonial affairs, had triggered the revolt. By 1770 the movement for independence had gathered substantial following, making it a full blown war against Britain. Colonists gathered in huge numbers and participated in protestations. They applied paints on their faces, wore Mohawks as a mark of identifying with America, while simultaneously distinguishing themselves from British troops. It was at this juncture that the famous Boston Tea Party happened. Acting in disobedience to the orders given from England, the colonists marched down onto the ships that sat in the bay that contained crates of tea. After